Download Advances in Tracer Methodology: Volume 4: A collection of by Waldo E. Cohn (auth.), Seymour Rothchild (eds.) PDF

By Waldo E. Cohn (auth.), Seymour Rothchild (eds.)

The 11th Symposium on Advances in Tracer technique­ ology was once held in Boston on October 13-14, 1966. The sym­ posium, which was once subsidized via the recent England Nuclear company, honored the 10th anniversary of the company. The Symposia on Advances in Tracer method, that have been held on a regular basis considering 1957, collect learn employees in numerous disciplines who proportion an curiosity in radioactive tracers. The 4 volumes, this one representing the list of the 11th Symposium, offer a compact and on hand resource of information-much of which was once often scattered through the clinical literature-of inter­ est to biochemists, pharmacologists, endocrinologists, and analysts for whom tracers became an imperative device. The editor is mainly thankful to Dr. Konrad Bloch, Harvard collage, and Dr. Waldo E. Cohn, Oak Ridge nationwide Laboratory, for chairing the classes on the 11th Sym­ posium. The cooperation of the audio system is deeply liked. additionally, a unique bouquet to my secretary, Mrs. Edith Thompson, for her beneficiant advice in arranging many of the symposia and the ebook of the papers. S. R.

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J. Am. Chern. Soc. 76:5162 (1954). 31. , Tetrahedron Letters 3351 (1964). 32. , Australian J. Chern. 15:56 (1964). 33. , Australian J. Chern. 16:549 (1963). 34. , J. BioI. Chern. 149:505 (1943). 35. , J. BioI. Chern. 198:861 (1952). 36. , J. Am. Chern. Soc. 75:1992 (1953). THE PREPARATION OF N-METHYL-C 14 LABE LED DRUGS Robert E. McMahon and Frederick J. Marshall Lilly Research Laboratories Indianapolis, Indiana In modern pharmaceutical research practice an everincreasing emphasis is being placed on studies of drug dynamics, that is, the absorption, distribution, metabolism, and elimination of an administered drug.

5% (wt/vol) trichloroacetic acid, 90 to 95°C, 30 min 6. Alcohol, ether, and dried The final residue is mainly denatured protein. 5 N HCI at no°C for 24 hr. As high as 75 to 80% of the total C 14 in the hydrolysate can be accounted for in Pincus Peyser 47 the fifteen pure amino acids isolated. Allowingfor losses, and breakdown during hydrolysis, it is estimated that as much as 90% of the residue before hydrolysis may be protein. Of the eighteen commonly occurring amino acids, threetryptophan, methionine, and cysteine-are present in Chiorella protein in relatively small amounts [12] and usually do not survive acid hydrolysis.

39. S •• J. Am. Chern. Soc. 52:419 (1930). 40. , Chern. Pharm. Bull. (Japan) 14:1007 (1966). 41. , Pharmacol. Rev. 18:95 (1966). 42. , J. BioI. Chern. 237:1657 (1962). 43. , Anal. Biochem. 16:349 (1966). 44. , J. Pharm. Sci. 55:457 (1966). 45. Tolbert, B. , and Cozzetto, F. , in: Preparation and Bio-Medical Application of Labeled Molecules, Euratom, 1964. 46. Okita, G. , in: Isotopes in Experimental Pharmacology, University of Chicago Press, Chicago, 1965. 47. , The Biological Disposition of Morphine and Its Surrogates, World Health Organization, Geneva, 1960.

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