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Cultivation of a common cold virus in organ culture. Brit. Med. , i, 76. 22. Webster, L. T. (1933). Summarized by Wilson, G. S. and Miles, A. A. , Baltimore. 23. , Hill, A. , Topley, W. W. C. and Wilson, J. (1936). Experimental Epidemiology, Special Report Series N o . 209, Medical Research Council, London. TABLE Preparations tested for storage 1 of micro-organisms at reduced temperatures Agar 0-2% Ascorbic acid 001% Casein, isoelectric 2% Casein, purified 27c Casein, technical 27c Caseinate, sodium 27c a Gelatin 0 - 5 % , 2 % , 57c a Gelatin (27c) + skim milk ( 5 % ) Gelatin phosphate diluent a a 10%, 2 0 % , 5 0 % Horse serum Horse serum ( 2 5 % ) + s k i m milk (57c) Lactalbumin 27c Lactalbumin hydrolysate 27c Nutrient broth 1-5%, 37c Parenamine a Peptonized milk 3% Proteose peptone 0-2% Saline Skim milk 0 - 5 % , 5%, 10% Skim milk (5%)+gelatin ( 2 % ) + s t a r c h (0 0 5 % , 0 1 % , 0-5%, 1%) Skim milk (5%)+gelatin ( 2 % ) + h o r s e serum ( 1 0 % ) + s t a r c h (0 0 5 % , 0 1 % , 0-5%, 1%) Stuart's amino acid 1-5%, 3 % Stuart's medium Thioglycollate a Preparations giving 90 to 100% recovery after 6 weeks at — 7 0 ° C .
Associated with the increased mortality is prolonged retention of intrapulmonary bacterial viability, a shortening of the lag phase and an increased rate of bacterial multiplication. The diminished lag phase and enhanced bacterial growth contribute to the slowing of the so-called bacterial clearance rate. Bacterial invasion of the blood appears to take place between the second and fourth days inclusively and equates with subsequent mortality. Bacteria present in the blood are in insufficient numbers to appreciably affect the total lung counts.
E. slowing of the clearance rate and enhancement of mortality, are related, such association having been previously noted for alcohol administration, and exposure to nitrogen dioxide and ozone (3, 4, 6). Bacterial clearance is generally thought to be a summation of those factors which physically remove bacterial cells from the lung and those which cause their loss of viability in situ. Although there is some difference of opinion as to the relative Alterations of the pathogenic role of streptococci group C in mice 55 importance of these two factors in this phenomenon, recent work in mice, at least, indicates that the decline in bacterial viability plays the major role (7).